Fig 1: IGFBP7 promotes TAM infiltration by activating FGFR1/PI3K/AKT signaling.A Differentially expressed genes of macrophages polarized towards M1 phenotype or towards M2 phenotype. B FGFR1 was significantly downregulated in M1 macrophages but upregulated in M2 macrophages. C, D The GSEA and expression correlation analysis showed that IGFBP7 was highly associated with PI3K/AKT signaling in GC. E Exogenous IGFBP7 promotes TAM/M2 macrophages polarization via FGFR1/PI3K/AKT signaling. F High level of M2/TAM macrophage infiltration predicted poor prognosis in GC. **p < 0.01.
Fig 2: IGFBP7 depletion suppressed FGF2 expression and secretion in GC.A The differentially expressed genes after IGFBP7 depletion were shown in the heatmap. Among them, FGF2 was downregulated by knockdown of IGFBP7 in GC. B The expression correlation analysis between protein-coding genes and IGFBP7 was conducted in GC. C A total of 17 genes that co-expressed with IGFBP7 were greatly downregulated by IGFBP7 depletion in GC cell lines. D, E IGFBP7 was highly co-expressed with FGF2 in both GC tissues and normal stomach tissues. F, G RNA-seq and qRT-PCR analysis showed that IGFBP7 depletion decreased the expression of FGF2. H, I ELISA assay verified that IGFBP7 knockdown decreased secreted level of IGFBP7 and FGF2. **P < 0.01.
Fig 3: The clinical value of IGFBP7 was analyzed in ACRG cohort.A The expression difference of IGFBP7 in intestinal and diffuse GC. B The expression difference of IGFBP7 between GC patients with/without perineural invasion. C, D The expression difference of IGFBP7 in GC patients with different pathologic stages or Borrmann stages. E–G The expression level of IGFBP7 in GC tissues with different TNM stages. H The expression difference of IGFBP7 between GC patients with pylorus-sparing radical gastrectomy (STG) operation or total gastrectomy (TG) operation. I, J The overall-survival and disease-free survival analysis of IGFBP7 in GC. K The expression difference of IGFBP7 between GC patients with/without MLH1 expression. L The expression difference of IGFBP7 between GC patients over 55 years old and patients under 55 years old. **P < 0.01.
Fig 4: The prognostic significance of IGFBP7 in GC was analyzed in TCGA cohort.A IGFBP7 expression in 27 paired GC samples in TCGA_STAD datasets. B IGFBP7 was overexpressed in GC. C The expression difference of IGFBP7 in intestinal and diffuse GC. D IGFBP7 expression was positively correlated with poor differentiation of GC. E–H The expression level of IGFBP7 in GC tissues with different TNM stages and pathologic stages. I The expression difference of IGFBP7 in GC patients with/without H. pylori infection. J GC patients with Barrett’s esophagus possessed relatively low expression of IGFBP7. K, L The overall-survival and disease-free survival analysis of IGFBP7 in GC. **P < 0.01.
Fig 5: IGFBP7 expression was positively regulated by EMT signaling in GC.A Gene expression correlation analysis confirmed that IGFBP7 was related to EMT signaling in GC. B, C Expression level of IGFBP7 in the different subtypes of GC in GSE35809 and GSE62254 cohorts. D The expression of IGFBP7 was determined by qRT-PCR after TGF-beta treatment. E, F The expression correlation between IGFBP7 and OVOL2 in GC tissues and normal stomach tissues. G RNA-seq analysis was conducted in GC cells overexpressing OVOL2. IGFBP7 expression was decreased in GC cells overexpression OVOL2. H Quantitative RT-PCR assay showed that the expression of IGFBP7 was significantly reduced by OVOL2 in GC. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05.
Supplier Page from Abcam for Recombinant Human IGFBP7 protein